This is a list of the enemies present in the game.
- Ant Troopers
- Ball n' Chains
- Biddybuds
- Big Boos
- Boos
- Cat Bullet Bills
- Cat Goombas
- Chargin' Chucks
- Charvaarghs
- Cheep Cheeps
- Conkdors
- Fire Bros
- Flaptor
- Goombas
- Goomba Towers
- Hammer Bros
- Magikoopas
- Missile Bills
- Para-Biddybuds
- Piranha Creepers
- Piranha Plants
- Piranha Sprout
- Shy Guys
- Spikes
- Walleyes
Bosses
- Volcano Dragon
- Wingo
