During the Night, the use of mushrooms (counted as plants for entertainment purposes) becomes essential due to the lack of sunlight. The first mushroom you receive is the only plant in the game that costs no sun. Indeed, all of the plants received here are mushrooms, and tend to be lower in cost than plants (though they sleep during the daytime).
| Plant | Cost | Effect | Unlocked |
| Puff-Shroom | 0 | Fires spores at zombies up to three squares away. | 2-1 |
| Sun-Shroom | 25 | GIves 15 sun per 24 seconds, then grows to give 25. | 2-1 |
| Fume-Shroom | 25 | Shoots fumes at zombies up to four squares away | 2-1 |
| Grave Buster | 25 | Destroys gravestones | 2-1 |
| Hypno-Shroom | 25 | Confuses zombies into fighting each other | 2-1 |
| Scaredy-Shroom | 25 | Shoots spores at distant zombies, hides from nearby zombies | 2-1 |
| Ice-Shroom | 25 | Freezes all zombies on the screen briefly, dealing slight damage | 2-1 |
| Doom-Shroom | 25 | Explodes zombies in 35.5 square radius, damages ground for 180 seconds | 2-1 |
