Like the first game, there are animal buddies that can help the Kongs on their quest.
Rambi the Rhino - can ram enemies and has a supercharge for his horn allowing him to charge even faster as well as break through bonus doors.
Enguarde the Swordfish - helps underwater with his swimming ability. Can attack enemies and also has a supercharged attack for breaking through to bonus areas.
Squawks the Parrot - can attack by shooting eggs from his beak. A blue sub-species is found in the level "Parrot Chute Panic" and can only descend in the level.
Rattly the Rattlesnake - can spring up and jump really high.
Squitter the Spider - can shoot webs that can defeat enemies or be used as platforms.
Clapper the Seal - Jumping on clapper will make him cool down or freeze water found in his levels.
Glimmer the Anglerfish - illuminates dark areas.
Expresso II - only found in the Gameboy Advance version of the game, he is playable in the Expresso Racing minigame.