The characters are listed in the order in which they are unlocked throughout the story.
| CHARACTER 1 | CHARACTER 2 | UNLOCKED |
| Kogoro Tenzai | Mii Kouryuji | Available from beginning |
| Chun-Li | Morrigan Aensland | Chapter 1 |
| Akira Yuki | Pai Chan | Chapter 2 |
| Frank West | Hsien-Ko | Chapter 3 |
| Reiji Arisu | Xiaomu | Chapter 4 |
| Soma Schicksal | Alisa Ilinichina Amiella | Chapter 5 |
| Gemini Sunrise | Erica Fontaine | Chapter 6 |
| Zephyr | Leanne | Chapter 7 |
| Kite | BlackRose | Chapter 7 |
| Ryu | Ken Masters | Chapter 8 |
| Ichiro Ogami | Sakura Shinguji | Chapter 9 |
| Dante | Demitri Maximoff | Chapter 10 |
| Haken Browning | Kaguya Nanbu | Chapter 11 |
| Yuri Lowell | Estellise | Chapter 12 |
| Kurt Irving | Riela Marcellis | Chapter 13 |
| Jin Kazama | Ling Xiaoyu | Chapter 16 |
| Toma | Cyrille | Chapter 18 |
| KOS-MOS | T-elos | Chapter 19 |
| Chris Redfield | Jill Valentine | Chapter 21 |
| Mega Man X | Zero (Mega Man) | Chapter 27 |
